Introduction:
Denaturation is a process in wich proteins or nucleic acids lose the quaternary, tertiary and secondary structure that is present in their native state.
Denaturation is the result of the application of some external stress or compounds such as a strong acid or base, a concentrated inorganic salt or organic solvent.
Material:
2x250mL beaker
4 test tubes
Test tube rack
10mL Pipet
Knife
Glass marking pen
Potato
Distilled water
Hydrogen Peroxide
NaCl
HCL
Objectives:
- Study the relation between the structure and the function of proteins
- Understand how temperature, pH and salinity affect to the protein structure.
Procedure:
Catalase is a common enzyme found in nearly all-living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen perioxide (H2O2) to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage and preventing the accumulation of hydrogen peroxide.
In this experiment we are going to test the catalse activity in different environment situations. We are going to measure the rate of enzyme activity under various conditions, such as different pH values and temperatures. We will measure catalse activity by observing the oxygen gas bubbles when H2O2 is destroyed. If lots of bubbles are producted, it means the reaction is happening quickly and the catalase enzyme is very active.
1. Prepare 30 ml of H2O2 10% in a beaker (use a pipet) --> 10g H2O2 90g H2O
2. Prepare 30 ml of HCL 10% in a beaker. --> 10g HCl 90g H2O
3. Prepare 30 ml of NaCL 50% in a beaker. --> 50g NaCl 50g H2O
4. Peel a fresh potato tuber and cut the tissue in five cubes of 1cm3. Weigh them and equal the mass.
5. Label 5 test tubes (1,2,3,4,5)
6. Inmerse 10 minutes your piece of potato inside HCl beaker
7. Inmerse 10 minutes another piece ofpotato inside NaCl beaker
8. Boil another piece of potato
9. With a mortar, mash up the third piece of potato
10. Prepare 5 test tubes as indicated below:
Observations:
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