Introduction:
In this lab experiment we are going to identify and differentiate the animal and plant cells. The food that we will use are onion and allium porrum (vegetal cells). And to see the animal cells we scrape the inner side of the cheek using a toothpick.
Material:
- Toothpick
- 2 slides
- 2 covers slips
- Distilled water
- Methylene blue
- Iodine
- Onion
- Glycerine
Objectives:
- Identify the major components of cells.
- Differemtiate between animal and plant cells.
- Measure dimensions of the entire cell and nucleus.
Procedure:
(PLANT CELLS OBSERVATION)
1. Pour distilled water into a watch glass.
2. Peel off the leaf from half a piece of onion and using forceps, pull out a piece of transparent onion peel (epiderms) from the leaf.
3. Put the epiderms in the watch glass containing distilled water.
4. Take a few drops of iodine solution in a dropper and transfer into another watch glass.
5. Using a brush, transfer the peel into the watch glass containing the dye. Let this remain in the iodine solution for 30 seconds.
6. Take the peel from the iodine solution and place it in the watch glass containing distilled water.
7. Take a few drops of glycerine in a dropper and put 2 or 3 drops at the center of a dry glass slide.
8. Using the brush, place the peel onto the slide containing glycerine.
9. Take a cover slip and place it gently on the peel with the aid of a needle.
10. Remove the extra glycerine using cellulose paper.
11. Finally, view in the microscope the result
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